Study the Effect of Bacteriophage on Biofilm Producer Pseudomonas aeruginosa causing Wounds and Burn Infections
Abstract
Pseudomonas aeruginosa is one of the opportunistic bacterial pathogens that has a great tendency to develop resistance to many types of antibiotics, and contributes to its pathogenicity and danger to the host , its possession of several virulence factors such as The biofilm, the bacteria have a biofilm become highly resistant to immune system and antibiotic treatment, therefore, it is believed that treatment with phages can be effective for eliminating the biofilm and thus reducing the risk and virulence of bacteria. One hundred clinical samples of wounds and burns were collected from Baghdad city hospitals for the purpose of outwardly identification it and observing the bacteria on the culture in terms of its color, shape and distinctive smell in addition to its ability to produce pigment, and to identification it microscopically, it was stained with Gram stain and biochemical tests (Catalase, Oxidase, IMVC), and final identification by using VITEK2 system. In this study, 42 clinical isolates of P. aeruginosa were obtained after phenotypic, microscopic identification, using VITEK2 system to final identification. Their antibiotics resistance was tested by determining the Minimum Inhibitory Concentration (MIC). It showed different levels of resistance. The results showed the high sensitivity of the biofilm towards the bacterial phage and its ability to disperse its biomass, compared to the detection results before treatment. High inhibition rates were recorded for the biofilm. The results of detection before treatment were 42.85%. With strong composition, 50% with medium composition, and 7.14% with weak membrane formation, but it showed a difference in the percentages after treatment, as the percentage of 4.76%, that is, only two isolates, maintained its effectiveness in forming biofilm at a strong level, while the rest of the isolates ranged between 57.14% with medium composition. Also, 38.09% are weakly formed weak.
