Prevalence and Antimicrobial Susceptibility Patterns of Bacteria Isolated from Body Fluid Specimens

Abstract

The current study carried out to determine the predominance of bacterial isolates responsible for body fluid infections and determine the antimicrobial resistance patterns of all bacteria which isolated in this study during a period extended form the first of January, 2021 to the end of December, 2021 at Teaching Laboratories in the medical city. During this study, 212 body fluids specimens (synovial, pleural, ascetic, Cerebra spinal fluid (CSF), seminal, peritoneal, BAL, nasal and bronchial fluids) were collected from both sexes and all ages. Bacterial isolates were isolated according to standard microbiology methods, then the identification of bacteria was done by Vitek-II system. Out of these 212, the highest percentage was from ascitic fluid 72(33.96%) while the lowest percentage was from nasal fluid 2(0.94%). On the other hand 81 (38.21%) of specimens showed positive bacterial growth, while 131 (61.79%) of specimens showed negative bacterial growth. Out of 81 positive bacterial growth, 51(63%) of the bacteria were Gram-positive and 30(37%) of the bacteria were Gram-negative. Out of those 51 isolates of the gram positive bacteria, Staphylococcus epidermidis isolates were predominant 9(17.65%), followed by Staph. hominis ssp hominis 8(15.68%), while Burkholderia capacia isolates 7(23.33%) were the predominant gram negative bacteria followed by isolates of Escherichia coli 5(16.67%). Isolates of Gram negative bacteria including Burkholderia capacia, Escherichia coli, Salmonella, Acinetobacter baumannii, Pseudomonas and Entereobacter showed highest resistance rate toward Ampicillin, Aztreonam, Cefazolin and Ceftriaxone and the highest sensitivity rate was toward Meropenem and Imipenem. Isolates of Klebsiella pneumoniea ssp. Pneumoniae, Salmonella entrica and Acinetobacter baumannii isolated in this study were multi-drug resistance and they resist to all antimicrobial agents used in this study.