Effect of Bio-synthesize Selenium Nanoparticles from E. coli Bacteria on Biofilm of Pathogenic Microbes

Abstract

The selenium nanoparticles are known to exhibit diverse biological properties such as antibacterial, antifungal, and antibiofilm. The study aims to biosynthesize selenium nanoparticles using Escherichia coli and evaluate their antimicrobial and antibiofilm efficacy. In this study, selenium nanoparticles was biosynthesized from the precursor (sodium selenite) using Escherichia coli (65) isolates, and  the biofabricated nanoparticles were characterized employing an array of techniques including UV–visible spectroscopy (at 266 nm), X-ray diffraction (at 2θ of 27.605 , 32.092, 45.652, 56.815). Scanning electron microscope (39.5-50.7 nm) and Fourier transform infrared spectroscopy; also the Dynamic Light Scattering analysis measured the size distribution and poly dispersity index (PDI). The data also revealed that E.coli Se-NPs effectively inhibited the growth of pathogens (Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Candida albicans), also, the highest inhibition zones (21, 20 and 19 mm) was noticed to Candida albicans, Klebsiella pneumoniae and Staphylococcus aureus respectively. The inhibition and degradation of bacterial biofilm were studied against all the tested strains (S. aureus, P. aeruginosa, Klebsiella pneumoniae , E. coli and Candida albicans. The antibiofilm against all pathogenic microorganisms were strong inhibition and the antibiofilm activity of SeNPs were statistically significant at concentrations of  Minimum inhibitory concentration (MIC) and sub MIC μg/mL (∗P value <0.05), and this was further confirmed by scanning electron microscopy (SEM). The current study concluded that E.coli- SeNPs could be used to prepare biological antimicrobial and antibiofilm agent effective against major pathogenic microbes.