Molecular Detection of InvA and Sop Genes in Salmonella spp. Isolates from Stool Samples of some Iraqi Patients

Abstract

Salmonella typhimurium is the main cause of gastrointestinal diseases for the human population and one of the most dangerous foodborne bacteria. This study aims to detect the occurrence of different important resistance genes in Salmonella typhimurium. Stool samples were collected from some hospitals in Baghdad-Iraq from different patients for bacterial isolation. 110 samples aggregate and identified by morphological tests and affirmed using the vitek-2 system. Antibiotic sensitivity tests of fourteen isolates belonging to clinical samples were spread by agar diffusion method. The result showed that Salmonella showed the diameters of the inhibition zones for 7 antibiotics and the antimicrobial Susceptibility rate of 14 Salmonella Isolates while a genomic DNA kit was used to extract bacterial genome from isolates then screened for virulence and resistance genes mainly InvA and Sop performed by polymerase chain reaction (PCR). In addition, the study showed that InvA  and Sop genes are found in all the 24  isolate which means even asymptomatic patients had resistance genes.