Molecular Detection of fur and Iron Responsive Genes in Local Isolates of Pseudomonas aeruginosa from Wounds and Burns

Abstract

Fourty samples were obtained from burns and wounds, from various ages. specimens were collected through the period extending from February to December 2022, from patients were admitted in AL-kindy hospital,Alnumaan teaching hospital,Ibn-albaladi for women and children and Imam Ali hospital in Baghdad. Identification of isolates were performed by biochemical and cultural tests, the results revealed that all isolates were P. aeruginosa. Conventional PCR (Polymerase Chain Reactin) was also used to confirm these isolates as P. aeruginosa by detection of 16 SrRNA but the results showed that this gene was located in only 30(75%) out of 40 biochemically P. aeruginosa isolates. PCR was also used to screen fur gene and iron responsive genes which are (pvd ,pch ,exoS and exoA) of the thirty isolates of P.aeruginosa and  results revealed that exoS gene was located in only 14(46.6%) out of 40 isolates, while for pvd gene results revealed only 21(70%) of isolates were consist of this gene out of 30  P. aeruginosa isolates, for pch gene 15(50%) of isolates were consist of  this gene out of 30  P. aeruginosa isolates, 20 (66.6%) were appeared to have for exoAgene out of 30  P. aeruginosa and 27(90%) of isolates were positive for fur gene. The source of these isolates which was burn swab n=20 (66.6%) and wound swab n =10 (33.3%).