Inhibitor Effect of Cinnamomum cassia Hexane Extract- Chitosan Nanoencapsulated on fimA and mrkA Biofilm Genes in Klebsiella Pneumoniae Isolated from Urinary Tract Infections

Abstract

Among the phytochemicals extracted from Cinnamomum cassia bark using hexane, which revealed the presence of terpenoids, alkaloids, flavonoids and phenols. Once 100 Klebsiella isolates from 170 UTI samples were morphologically cultured, only 60 Klebsiella pneumoniae isolates could be identified using conventional bacteriological and morphological methods. A highly pure RNA sample was collected. The three K. pneumoniae isolates were tested for fimA and mrkA gene mRNA expression compared with rrsE gene (housekeeping) using RT-qPCR. The RT-qPCR results show that when exposed to subMIC CHEx and CHCsNPs, respectively, the expression of the fimA gene significantly decreased (p≥ 0.05) as compared to the control group. This study utilizes quantitative RT-qPCR screening of the mrkA gene to uncover variations in gene expression among three distinct K. pneumoniae isolates. The mrkA gene Ct value ranging from 6.0 to 8, and did not differ statistically according to the analysis. K. pneumoniae bacteria secrete a many toxin, because the mrkA gene was not strongly expressed.