Estimation of JAK2 Serum Level and Gene Expression in Diabetes Type 2 Patients

Abstract

Type 2 diabetes mellitus (T2DM) is a multifactorial not-communicable disease that is characterized by insulin resistance and chronic sub-clinical inflammation.  This research aimed to study the role of biochemical and immunological marker (IL23) level its effect on the gene expression of JAKs in T2DM. The study divided participants into two groups: 60 with T2DM and 60 control aged 35-74 who attended Sheikh Zayed Hospital from Nov 2023 to Jan 2024. Participants underwent blood sampling to extract RNA for JAK2 transcript analysis using cDNA synthesis in RT-PCR, additionally the research also assessed the clinicopathological traits of diabetic patients and IL-23 concentration using ELISA. Type 2 diabetic patients showed significantly high JAK2 expression. The study found a correlation between IL-23 concentration, JAK2 expression level, and they may act as clinicopathological aspects like HBA1C and FBS levels. In conclusion, T2 DM had elevated JAK2 and IL-23 levels Therapeutic targets of the disease linked to disease activity and pathophysiology may exist.