Study the Relationship of Biochemical and Immunological (IL-23) Level and SOCS3 Expression in Diabetes Type 2 Patients

Abstract

diabetes mellitus, a metabolic disorder distinguished by chronically high blood glucose levels, includes thirst, weight loss, and excessive urination. In diabetic patients, there is a correlation between the overexpression of the cellular signaling inhibitor SOCS3 and both insulin resistance and glucose levels. The present research aims to examine the impact of IL-23 on the expression of the SOCS3 gene. In the present study, 120 individuals were Involved between November 2023 and January 2024, 60 of whom were diagnosed with Type 2 Diabetes Mellitus (T2DM) treated with Glucophage and Amaryl, excluding patients who use sitagliptin as a treatment `and the remaining 60 were apparently healthy. To evaluate the level of SOCS3 Each and every individual had blood samples taken and RNA was extracted. cDNA synthesis was performed, and real-time PCR was used for analysis. Compared to healthy individuals, SOCS3 gene expression was upregulated in T2DM patients (fold change: 3.75). Moreover, T2DM patients had elevated IL-23 levels, suggesting a correlation with disease activity.in conclusion, our findings indicate that SOCS3 expression and IL-23 are positively linked with insulin resistance indicators in a diabetic environment.