Propagation GAC Fruit Plants in Vitro and Study Their Stability by ISSR Markers

Abstract

Genetic Stability analysis of in vitro cultivated GAC fruit (Momordica cochinchinensis Spreng) from the Horticulture Department station using ISSR markers. These are difficult to apply for breeding, resulting in limited availability. Resulting in a lack of seed availability. However, tissue culture allows the development of micropropagate variations, especially in plantlets undergoing several subculture stages. This study aimed to identify somaclonal variations in GAC fruit seedlings generated from in vitro culture using Inter Simple Sequence Repeat (ISSR) markers. Two accessions were used in this study consisting of GAC fruit grown in its original environment and in vitro cultured. Total of five primers, of which four primers detected polymorphisms among the population. This study resulted in a somewhat similar genetic similarity among the GAC fruit. The genetic diversity of GAC fruit plants propagated by in vitro culture had a similarity level of 92%, showing somaclonal variations in vitro culture