Generation of STR Profile From Touched Glass Surface

Abstract

Crime scene investigation involves analysis of surfaces for criminal traces. Touch DNA analysis now one of the essential tests performed for criminal identification. The aim of this study was to investigate the possibility of generation STR profile from touched glass surface. Window glass surface was touched with clean hand for 15 seconds then a double buccal swab methods used to collect possible skin cells. DNA extraction was performed using Chelex method then quantified by real time PCR. Quantified DNA amplified by STR kit (Minifiler) then analyzed using ABI 3130XL genetic analyzer. The results showed that the extracted DNA was quantified and analyzed successfully to give intact STR profile. These results indicated the capability of generating full DNA STR profile from glass surface using Chelex method.