Screening for TKD mutation in fms-like tyrosine kinase 3 (FLT3) gene in Iraqi patients with acute myeloid leukemia

Abstract

Acute myeloid leukemia (AML) is a destructive hematological tumor illness marked via uncontrolled proliferations of myeloid progenitor cells in the bone marrows. One of the most common genetic variations in AML is the mutations in the FLT3 gene. This study aimed to discover the mutations in the tyrosine kinase domain (TKD) of the FLT3 gene. This study consisted of two groups, the control group included 50 apparently healthy subjects and 50 newly diagnosed Individuals with AML. Blood samples were collected for DNA extraction used in the RFLP to detect the TKD mutation. The Eco RV enzyme was used to detect the TKD. The wild-type fragments were 80 bp, 51 bp, and 20 bp, while the mutant-type fragments were 131 bp and 20 bp. The findings indicated that the ratio of the TKD mutation was significantly (p˂0.05) greater in males than in females. All the TKD mutations detected in the present study were heterozygous. The TKD mutation ratio was 8 % in Iraqi Individuals with AML. According to the FAB classification, the mutant cases were 3 in the M5 and 1 in the M7. WBC was significantly (P=˂0.01) greater in Individuals with AML than in the healthy controls (HCs). No significant variations were noted between the wild-type and the mutant-type related to the WBC count and peripheral blast cell in patients.