Evaluation the Levels of Stem Cells Markers in the Cultured Cells Isolated from Human Chorionic Plate of the Fetal Side

Abstract

Stem cells are exclusive population of cells located in all stages of lifetime that holds capability to self-renewal plus specialize to group of cell lineages of multiple organs. Stem cells within human chorionic tissues consider have potential applications because they serve as a source of multipotency for multiple cells regeneration. These cells express certain surface markers for their detection includes CD90, CD105, CD73, CD34 and CD14,  Samples were collected from newly baby born placenta. Stem cells enrichment was conducted using enzymatic method of collagenase type I and culturing using DMEM-low glucose for several days. The results showed that the collected cells after culture demonstrated increased level of gene expression for targeted markers mentioned above by RT-PCR in comparison with the cells.

In conclusion: the isolated cells demonstrated increase in the expression of stem cells markers after culture, which indicate stem cells self-enrichment after day six of culture.