Expression of Catsper Genecombination in Improves Sperm Parameters in Vitro of Normozoospermic Men with Anti Sperm Antibody Factor
Abstract
Such a defect, any such one that causes infertility of the CATSPER cation channel, a particular calcium channel of the sperm tail, results in hyper-activation of sperm motility. In an attempt to enhance some of the sperm characteristics that are relevant by ART clinics, this research aims to investigate the relationship between CATSPER Channele and anti-sperm antibodies in Normozoospermic men before and after in vitro activation. From September 2024 to February 2025, 75 semen samples of men were received at Kamal Al-Samarrai Hospital for Fertility and Infertility's Andrology Laboratory, Bagdad, Iraq. 50 normal viable controls and 25 normozoospermic men with Antisperm anti body (ASA) were considered after seminal fluid analysis. In vitro sperm activation by stacking technique and FertiCultTM Flushing media was used in all the semen samples. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was applied to quantify CATSPER gene expression in semen samples. Statistical Package for Social Sciences (SPSS) software version 23.0 was applied for statistical analysis. Descriptive statistics such as mean, standard error, frequency, and range were employed, and Students t-test was applied for data comparison between treatment and control groups. Mean ± SEM was applied to represent treatment and control group data. All analyses indicated a highly significant (p< 0.001) increase in active grade A sperm motility, expression of down-regulated genes after the layering method, and highly (p<0.001) progressive motility % and significantly enhanced MNS compared to the control group, whose level of gene expression did not exhibit non-significant difference upon activation (P>0.05). In vitro activation method of layering should be applied to sperm activation and preparation in ART programs, according to the current study, since it has the ability to improve sperm quality in male infertility.
