Evaluation of Gene Expression of AQP-1 Gene in Chronic Myeloid Leukemia of Iraqi Patients

Abstract

Chronic myeloid leukemia is a type of cancer characterized by myeloproliferative disorder in the hemopoietic stem cell (HSC) compartment. It is characterized by the overproduction of myeloid cells which are a type of white blood cell. This study aimed to evaluate the expression levels of the aquaporin-1 (AQP1) gene in Iraqi patients diagnosed with chronic myeloid leukemia. The study was conducted on 40 subjects, aged 8 to 63 years. Blood samples were collected from July/2024 to October/2024 in Baghdad, divided into 20 patients with CML (12 females, 8 males) and 20 healthy controls (12 females, 8 males) attending the Baghdad Private Lab. AQP1 genes were assessed using qRT-PCR. AQP-1 gene expression revealed that the outcome of the fold value in patients was 28.2 compared to the control 24.1, and the relative expression (2-∆∆ Ct) for the same gene was decreased (downregulation), which was 0.80. These results suggest that reduced expression of the AQP1 gene in chronic myeloid leukemia patients could have implications for fluid regulation, kidney function, and disease management. Further studies are needed to clarify the mechanisms underlying decreased AQP1 expression in leukemia and its clinical significance