The Impact of Tecoma stans ethanolic Extract on Gens Expression of MexA and MexB of Pseudomonas aeruginosa.

Abstract

An important therapeutic challenge in clinical settings is Pseudomonas aeruginosa a common opportunistic bacterium linked to drug resistance. Pseudomonas aeruginosa contains MexA and MexB as part of its MexAB-OprM efflux pump. This pump aids in the bacteria natural resistance to a variety of antibiotics. This efflux pump contributes to bacterial resistance to antibiotics. Finding out if the ethanolic 70% extract of Tecoma stans altered the expression of the genes encoding these efflux pump proteins was the aim of this study. Regarding clinical isolates of P. aeruginosa employing RT-qPCR and MIC to assess the gene expression of the ethanolic extract of the dried leaves of Tecoma stans. The results showed that the treated groups levels of MexA and MexB gene expression were significantly lower than those of the untreated control group. This suggests that by blocking the efflux pump Tecoma stans extract can increase the effectiveness of drugs against the resistant strain. Tecoma stan components may naturally block the mechanisms causing bacterial multidrug resistance according to the study.