Association of Transforming Growth Factor Beta1 Gene Polymorphism with Diabetes Mellitus Risk in Iraq Patients

Abstract

Diabetes mellitus (DM) refers to a group of multifactorial metabolic disorders characterized by elevated blood glucose levels (hyperglycemia) that result from defects in the body's ability to produce and/or insufficiency of insulin action .This study was to investigate the correlation between TGFβ1 polymorphism and Diabetes Mellitus .The present study carried out in the labs of college of education for pure science and Center for Diabetes and Endocrinology of the Health Directorate in Thi- Qar province, the period of research was extended from January- July 2017. To test for the association of Polymorphisms in promoter region (G-800A) and (C-509T ) of transforming growth factor- β 1 (TGF-β1) gene with diabetes mellitus in Iraqian patients. The study included a total of 120 patients with type I and II diabetes and their age between 1-51 years. in addition to 52 healthy controls . DNA has been isolated and RFLP-PCR was performed by using primers specific for  genotypes of two region of  the TGF β1 gene (C509T)and (G800A). The results showed that only C509T polymorphism of the TGFβ1 gene is significantly different in genotype distribution in allelic frequencies between DM patients and control subjects and association with clinical characteristics. Thus this SNP seems to be related to DM susceptibility. This study supports the involvement of TGFβ1 gene polymorphism in the incidence of DM in Thi-Qar population.