Association of C-allele carrier Genotype of SLCO1B1 gene 521T<C Polymorphism and Statins Related Myopathy in a Sample of Iraqi Patients

Abstract

The gene SLCO1B1 521 T>C is a crucial gene single nucleotide polymorphism (SNP) concerning of a several medications transport enzyme  OATP1B1 action variation such as statins drugs that may cause a serious side effect (myopathy), Iraq lack from recent studies about this variation related to statins side effect myopathy.The study of the SLCO1B1 gene 521 T>C polymorphism effect on Iraqis patients taken statins differ in showing myopathy as a side effect of statin. Settings and Design considered An only treated case-control study. The study of the effect of T521C of SLCO1B1 gene polymorphism and myopathy carried by collect venous human blood (3) ml of (96) samples divided to (48) as control and (48) as case. DNA extraction carried by (wizibiosolutions Korean kit for DNA extraction) and detection by Gel-Electrophoresis, nanodrop device used to adjust purity and concentration proper to next step, TaqMan Real-Time Polymerase Chain Reaction (TaqMan RT-PCR) carried by Real-Time PCR System . Myopathy determined by a history of the patient, clinical examination. Highly significant difference p-value  (0.0001>0.01) in genotype carry C-allele between control and case. Genotype (CC) was zero in control compared with cases, all (CC) genotype 5 patients with myopathy was (100%) in case. Genotype (TC) only 5 patients represent (15.6%) was in control parallel to 27 patients represent (84.4%) in case. Genotype (TT) wild type was 43 patients (72.9%) in control compare to only 16 case patients represent (27.1%). All numbers refer to a strong correlation between genotypes carry C-allele (100%, 84.4%) and case (patients with myopathy) against control only (15.6%) of (TC) genotype without myopathy. The significant  risk of myopathy of (CC) genotype compare with (TT) genotype when (n=96) odd ratio (17.2) at (95%) Cl between (5.7051 to 51.8551) and P (0.0001) indicate the risk of development of myopathy for C-allele carrier genotype. C-allele considered a risk factor for patients taken statin lead to develop of myopathy in Iraq.