Extraction and Quantification of DNA from Touched Cell Phones Surfaces

Abstract

Touched mobile surfaces became part of crime scene evidences which analyzed by forensic scientists. The minimal amount of DNA required for full STR profile is 0.2-0.5ng. The aim of this work is to quantitate the amount of DNA isolated from three touched mobiles brands (iPhone, Samsung, and Sony) surfaces using Organic and Chelex DNA extraction methods. The results showed that organic method extract more DNA than Chelex from Samsung mobile surface and fewer amounts from iPhone and Sony. Quantification of DNA by Real time PCR was more accurate with quantities near the minimal amount required for full profile analysis. STR analysis showed a partial DNA profile recovered from all cell phones brands. The results indicate the possibility of obtain suitable quantities of DNA from cell phone surface.