Gene Expression Profile of eNOS Gene in a Sample of Iraqi Asthenozoospermic Patients
Abstract
The expression of endothelial nitric oxide synthase (eNOS) gene represents one of the most important causes that effect the male infertility. The eNOS is primarily responsible that create of NO in the vascular endothelium, therefore eNOS is mainly expressed in the human testicular endothelial cells, in addition of Sertoli cells and Leydig, epididymis and vas deferens which regulates the synthesis of NO. Many study informed in the first time about the NO that is synthesized by human male gamete. Experimental evidence has shown that NO in high concentrations can cause defective function of the sperm and low concentrations of NO play very important role in the control of the sperm physiology. In this study, Approximately 50 Iraqi idiopathic asthenozoospermic (AZS) patients were recruited from Kamal Al-Samraee hospital, Baghdad, Iraq during a period from 15 November 2017 to 1 May 2018 and 50 healthy men were included for this study, Gene expression for eNOS of reverse transcription quantitative PCR (RT-qPCR) is distinguished from other methods because of its accuracy, sensitivity. Real time PCR quantification applied in the present experiment utilized the SYBR green, a fluorescent dye which recognizes any double stranded DNA including cDNA, the amplification was recorded as a Ct value (cycle threshold). The lower Ct value indicates the presence of higher copies of the target and vice versa. In terms of gene expression, high Ct values indicate low gene expression and low Ct value indicates a high gene expression . In this study we analyzed gene expression of eNOS genes by RT-PCR depending Ct value to measure gene expression in AZS patients and fertile. As related with eNOS genes the fold of gene expression in control was lower than AZS patients 4 times. Fold number in AZS patients were 4 times for eNOS gene. In calculation of the relative expression of eNOS gene in all study groups , the 2-ΔΔCt results were appliedو the gene expression in fertile lower than AZS patients 4.05 times for eNOS gene. Fold number in fertile was 1 time for eNOS gene.
