Evaluation of Crude Phenolic Extract of Alhagi graecorum Boiss Plant against Some Dermatophytes Isolated from Patients at Al-Yarmouk Teaching Hospital

Abstract

This study aimed to evaluate the crude  phenolic extract  from the Alhagi graecorum Boiss plant that contains a bioactive compound to treat dermatophytes. Eighty samples were taken from patients who underwent al-Yarmouk teaching hospital. plant extract  was performed by GC-Mass technology . Molecular diagnosis of  fungal isolation by PCR technique  were Trichophyton rubrum, Trichophyton mentographytes, and Microsporum canis . Results show that the fungicidal diameter for Trichophyton rubrum, Trichophyton mentographytes, and Microsporum canis was 20 mm at 3.125 mg/ml phenol. Fungi did not grow at 6.25, 12.5, 25, or 50 mg/ml phenol. Macroconidia and microconidia sporulation concentrations of Trichophyton rubrum at 3.125 mg/ml crude phenol were 1x10⁴ and 2x10⁴, respectively. At the same concentration, macroconidia and microconidia counts were zero at 2x10⁴ and 3x10⁴ of  Microsporum canis macroconidia, respectively. No significant sporulation was reported for macroconidia and microconidia of Trichophyton rubrum, Trichophyton mentographytes, and Microsporum canis at 6.25 mg/ml, 12.5 mg/ml, 25 mg/ml, and 50 mg/ml crude phenol concentrations. When the crude phenol content from Alhagi graecorum Boiss above 3.125 mg/ml, sporulation halted. In conclusion, the phenol crude isolated from Alhagi graecorum Boiss showed strong antifungal activity at concentrations above 3.125 mg/ml by lowering fungus diameter and preventing sporulation.