Evaluation of Crude Phenolic Extract of Alhagi graecorum Boiss Plant against Some Dermatophytes Isolated from Patients at Al-Yarmouk Teaching Hospital
This study aimed to evaluate the crude phenolic extract from the Alhagi graecorum Boiss plant that contains a bioactive compound to treat dermatophytes. Eighty samples were taken from patients who underwent al-Yarmouk teaching hospital. plant extract was performed by GC-Mass technology . Molecular diagnosis of fungal isolation by PCR technique were Trichophyton rubrum, Trichophyton mentographytes, and Microsporum canis . Results show that the fungicidal diameter for Trichophyton rubrum, Trichophyton mentographytes, and Microsporum canis was 20 mm at 3.125 mg/ml phenol. Fungi did not grow at 6.25, 12.5, 25, or 50 mg/ml phenol. Macroconidia and microconidia sporulation concentrations of Trichophyton rubrum at 3.125 mg/ml crude phenol were 1x104 and 2x104, respectively. At the same concentration, macroconidia and microconidia counts were zero at 2x104 and 3x104 of Microsporum canis macroconidia, respectively. No significant sporulation was reported for macroconidia and microconidia of Trichophyton rubrum, Trichophyton mentographytes, and Microsporum canis at 6.25 mg/ml, 12.5 mg/ml, 25 mg/ml, and 50 mg/ml crude phenol concentrations. When the crude phenol content from Alhagi graecorum Boiss above 3.125 mg/ml, sporulation halted. In conclusion, the phenol crude isolated from Alhagi graecorum Boiss showed strong antifungal activity at concentrations above 3.125 mg/ml by lowering fungus diameter and preventing sporulation.